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Super Apochromatic Objectives (SAPO) and Apochromatic Objectives (APO)

High Precision and High N.A. Custom Objectives

Shanghai Optics Super Apochromatic Objectives feature high sensitivity to fluorescence emissions, providing for images that are not only clear, but do not contain color shift. For the visible and NIR spectrum, these objectives correct for spherical and chromatic aberrations. Shanghai Optics Super Apochromatic Objectives feature high numerical apertures and high performance and quality. These water/oil immersion objectives are ideal for use in digital imaging of various needs, such as high-resolution observations deep inside living tissue.

SAPO and APO Product Features

Corrected from Visible to NIR Regions
High Sensitivity to Fluorescence Emissions
Extremely High Numerical Apertures
Deliver high performance observation

Application

For a variety of image applications, including, Epi-illumination, Focal illumination, Bright field and DIC (Differential Interference Contrast) Application. These special objectives have been widely used in medical, industrial, live cell imaging, fluoresce microscopy. The image has the appearance of a three-dimensional object under very oblique illumination, causing strong light and dark shadows on the corresponding faces. Even thought DIC observation only provides two dimensional image, due to the oblique illumination, the image still provides a three dimensional depth for observation.
These microscope objectives help to push the boundaries of knowledge and getting the most from our microscopy experiments.bThey can be used in a wide variety of applications. Such as Traditional multiphoton microscopy, con-focal imaging etc.

Samples of Custom SAPO and APO

The following are some samples of the custom SAPO and APO that we produced in recent years for your reference.

#1,3,5 These SAPO (Super Achromatic objectives) have diffraction-limited axial achromatic performance and a flat focal plane over a wide field of view and can be used in a garden variety of imaging techniques without vignetting. These SAPOs have a large numerical aperture (NA) and are ideal for wide field imaging. And transmission rate for fluorescence of these SAPOs are high, these objective lenses are used for qualitative and quantitative analysis of ion movement and for particularly critical of fluorescence microscopy (Such as human DNA research and cytogenetic studies)

The water immersion objective lens, together with the vertical microscope is mainly used for cell studies, epically for sample brain cell observation for thickness comparison.

#5, 6 Oil immersion objective, N·A=n·sinφ,

Oil immersion objective increases the refractive index of light pass through the medium and increases the NA (Numerical Aperture). These special custom objectives are widely used in biomedical engineering and research.

Item #1Plan SAPO 5x NA0.50
Magnification5x
Number Aperture(N.A)0.5
Working Distance(mm)2
Wavelength(nm)400-780
Field Number(mm)30
Thickness of cover glass(mm)0.17
ImmersionWater
Spring loadedNo
Correction ringNo
Correction level of PlanPlan
Correction level of chromatic                  aberrationSuper Apochromatism(SAPO)
Parfocal distance(mm)179.5
Back focal plane(mm)
Screw threadM25X0.75
Bright field(Transmission)Excellent
Fluorescence(B,G emission)Excellent
MultiphotonNo
Item #2GK Medical Plan APO 20x NA0.72
Magnification20x
Number Aperture(N.A)0.72
Working Distance(mm)4.2
Field Number(mm)24
Wavelength(nm)400-1100
Thickness of cover glass(mm)0
ImmersionWater
Spring loadedNo
Correction ringNo
Correction level of PlanPlan
Correction level of chromatic aberrationApochromatism(APO)
Parfocal distance(mm)60.06
Back focal plane(mm)
Screw threadM25X0.75
Bright field(Transmission)Excellent
Fluorescence(B,G emission)Excellent
MultiphotonNo
Item#3Plan SAPO 20x NA0.75
Magnification20x
Number Aperture(N.A)0.75
Working Distance(mm)1
Wavelength(nm)400-800
Field Number(mm)44
Thickness of cover glass(mm)0.17
ImmersionAir/Dry
Spring loadedNo
Correction ringNo
Correction level of PlanPlan
Correction level of chromatic aberrationSuper Apochromatism(SAPO)
Parfocal distance(mm)60.06
Back focal plane(mm)
Screw threadM30X0.75
Bright field(Transmission)Excellent
Fluorescence(B,G emission)Excellent
MultiphotonNo
Item#4Plan APO 20x NA0.80
Magnification20x
Number Aperture(N.A)0.8
Working Distance(mm)1
Wavelength(nm)400-800
Field Number(mm)30
Thickness of cover glass(mm)0.17
ImmersionAir/Dry
Spring loadedNo
Correction ringNo
Correction level of PlanPlan
Correction level of chromatic aberrationApochromatism(APO)
Parfocal distance(mm)60.06
Back focal plane(mm)
Screw threadM25X0.75
Bright field(Transmission)Excellent
Fluorescence(B,G emission)Excellent
MultiphotonNo
Item#5Plan SAPO 100x NA1.40
Magnification100x
Number Aperture(N.A)1.4
Working Distance(mm)0.13
Wavelength(nm)Visible
Field Number(mm)25
Thickness of cover glass(mm)0.17
ImmersionOil
Spring loadedYes
Correction ringNo
Correction level of PlanPlan
Correction level of chromatic aberrationSuper Apochromatism(SAPO)
Parfocal distance(mm)45.06
Back focal plane(mm) 
Screw threadWJ4/5″X1/36″
Bright field(Transmission)Excellent
Fluorescence(B,G emission)Excellent
MultiphotonNo
Item#6Plan APO 100x NA1.45
Magnification100x
Number Aperture(N.A)1.45
Working Distance(mm)0.13
Wavelength(nm)Visible
Field Number(mm)25
Thickness of cover glass(mm)0.17
ImmersionOil
Spring loadedYes
Correction ringNo
Correction level of PlanPlan
Correction level of chromatic aberrationApochromatism(APO)
Parfocal distance(mm)45.06
Back focal plane(mm) 
Screw threadWJ4/5″X1/36″
Bright field(Transmission)Excellent
Fluorescence(B,G emission)Excellent
MultiphotonNo
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