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Super Apochromatic (SAPO) Objective and Apochromatic (APO) Objective

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High Corrected and Large N.A. Custom Objectives

Shanghai Optics’ apochromatic microscope objectives provide highly optical corrections in a flat field of focus and have a high transmission from the visible to the near-infrared region.  These objectives are used in microscopy or imaging systems for delivering imaging quality that cannot be achieved by conventional objectives.

Shanghai Optics’ super apochromatic microscope objectives deliver diffraction-limited performance over the entire field of view from the visible to near infrared region. These objective lenses are designed for the most demanding research applications.

These objectives are designed for use in different immersion media such as air/dry, water or oil.

SAPO and APO Product Features

Highly Corrected from Visible to NIR Regions
Excellent Antireflection (AR) Coatings and High Transmission
Extremely High Numerical Apertures
Outstanding Optical Performance

Ideal for Brightfield and Fluorescence Imaging Applications

Application

The APO and Super APO objectives are ideal for use in life scientific research applications that require high sensitivity and outstanding imaging performance such as live cell imaging, deep tissue imaging, fluorescence imaging, etc.

Shanghai Optics offers SAP objectives and APO objectives in a range of magnifications for microscopy applications including Epi-illumination microscope, Bright field microscope, DIC (Differential Interference Contrast) microscope, phase contrast microscope, fluorescence microscope, etc. These objectives are designed to have higher numerical apertures and provide high performance for use in different immersion media such as air/dry, water or oil.

Samples of Custom SAPO and APO

In recent years many SAPO and APO objectives with large aperture and high transmission have been designed and manufactured for low light and widefield microscopy applications. The objectives shown below are some samples.

#2 and #4 objectives are apochromatic objective lenses that are corrected for both spherical and chromatic aberrations over the entire field of view. When comparing with conventional objectives, these two objectives have larger aperture and provide higher image quality in the visible and near infrared range.  #2 and #4 objectives have been used in high resolution microscopes and imaging systems for producing high contrast images without introducing vignetting. The large aperture and good quality make these APO objectives very useful for high resolution microscopy and imaging systems where the high sensitivity is needed for qualitative and quantitative analyses such as living cells, deep tissues, etc.

#1,#3 and #5, are super apochromatic objectives that are highly corrected for both spherical and chromatic aberrations and provide diffraction-limited optical performance at a flat focal plane without introducing vignetting. These objectives can be used in top quality microscopes and imaging systems for producing diffraction limited images without vignetting. These SAPO objectives are ideal for the most demanding research applications such as ion movement, human DNA research, cytogenetic study, etc.

In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the front lens element of the objective lens and the cover glass of the specimen in a transparent oil with high refractive index, thereby increasing the numerical aperture of the objective lens.

#5 and #6, are oil immersion objectives. These objectives with larger number aperture (NA) and high transmission are ideal for highly effective fluorescence imaging of living cells, tissues and other specimens.

Item #1 Plan SAPO 5x NA0.50
Magnification 5x
Number Aperture(N.A) 0.5
Working Distance(mm) 2
Wavelength(nm) 400-780
Field Number(mm) 30
Thickness of cover glass(mm) 0.17
Immersion Water
Spring loaded No
Correction ring No
Correction level of Plan Plan
Correction level of chromatic                  aberration Super Apochromatism(SAPO)
Parfocal distance(mm) 179.5
Back focal plane(mm)
Screw thread M25X0.75
Bright field(Transmission) Excellent
Fluorescence(B,G emission) Excellent
Multiphoton No
Item #2 GK Medical Plan APO 20x NA0.72
Magnification 20x
Number Aperture(N.A) 0.72
Working Distance(mm) 4.2
Field Number(mm) 24
Wavelength(nm) 400-1100
Thickness of cover glass(mm) 0
Immersion Water
Spring loaded No
Correction ring No
Correction level of Plan Plan
Correction level of chromatic aberration Apochromatism(APO)
Parfocal distance(mm) 60.06
Back focal plane(mm)
Screw thread M25X0.75
Bright field(Transmission) Excellent
Fluorescence(B,G emission) Excellent
Multiphoton No
Item#3 Plan SAPO 20x NA0.75
Magnification 20x
Number Aperture(N.A) 0.75
Working Distance(mm) 1
Wavelength(nm) 400-800
Field Number(mm) 44
Thickness of cover glass(mm) 0.17
Immersion Air/Dry
Spring loaded No
Correction ring No
Correction level of Plan Plan
Correction level of chromatic aberration Super Apochromatism(SAPO)
Parfocal distance(mm) 60.06
Back focal plane(mm)
Screw thread M30X0.75
Bright field(Transmission) Excellent
Fluorescence(B,G emission) Excellent
Multiphoton No
Item#4 Plan APO 20x NA0.80
Magnification 20x
Number Aperture(N.A) 0.8
Working Distance(mm) 1
Wavelength(nm) 400-800
Field Number(mm) 30
Thickness of cover glass(mm) 0.17
Immersion Air/Dry
Spring loaded No
Correction ring No
Correction level of Plan Plan
Correction level of chromatic aberration Apochromatism(APO)
Parfocal distance(mm) 60.06
Back focal plane(mm)
Screw thread M25X0.75
Bright field(Transmission) Excellent
Fluorescence(B,G emission) Excellent
Multiphoton No
Item#5 Plan SAPO 100x NA1.40
Magnification 100x
Number Aperture(N.A) 1.4
Working Distance(mm) 0.13
Wavelength(nm) Visible
Field Number(mm) 25
Thickness of cover glass(mm) 0.17
Immersion Oil
Spring loaded Yes
Correction ring No
Correction level of Plan Plan
Correction level of chromatic aberration Super Apochromatism(SAPO)
Parfocal distance(mm) 45.06
Back focal plane(mm)
Screw thread WJ4/5″X1/36″
Bright field(Transmission) Excellent
Fluorescence(B,G emission) Excellent
Multiphoton No
Item#6 Plan APO 100x NA1.45
Magnification 100x
Number Aperture(N.A) 1.45
Working Distance(mm) 0.13
Wavelength(nm) Visible
Field Number(mm) 25
Thickness of cover glass(mm) 0.17
Immersion Oil
Spring loaded Yes
Correction ring No
Correction level of Plan Plan
Correction level of chromatic aberration Apochromatism(APO)
Parfocal distance(mm) 45.06
Back focal plane(mm)
Screw thread WJ4/5″X1/36″
Bright field(Transmission) Excellent
Fluorescence(B,G emission) Excellent
Multiphoton No
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